|
KMID : 1007519990080050314
|
|
Food Science and Biotechnology
1999 Volume.8 No. 5 p.314 ~ p.317
|
|
|
Photoinduced Cytotoxicity of Polycyclic Aromatic Hydrocarbons in Mammalian Cells
|
|
Kim, Jong Sang
Park, Kwan Ha/Hwang, In Young
|
|
|
Abstract
|
|
|
|
The toxicities of anthracene, phenanthrene, and fluoranthene on the cultured animal cells were investigated. Inhibitory activities of cellular growth was dependent on the concentrations of polycyclic aromatic hydrocarbons (PAHs). IC_(50) values of PAHs for Hepalclc7 (mouse hepatoma) and CCD-986SK (human fibroblast) cells were 0.13-0.25 mM and 0.25-0.5 mM, respectively. Ultraviolet irradiation on the cells preincubated in the presence of different concentrations of PAHs resulted in an approximately tenfold increase in cytotoxicity, compared to the control cells subjected to PAH treatments alone. Cells incubated in the presense of anthracene showed much higher sensitivity to UVA (320-400 nm) than UVB (280-320 nm), while ultraviolet irradiation to cells pretreated with phenanthrene showed relatively low photoinduced toxicity. Fluoranthene showed a significantly high photoinduced toxicity regardless of the wavelength of ultraviolet used, with IC_(50) of 0.006-0.025 mM for both cell lines. Fluoranthene induced cellular arylhydrocarbon hydroxylase activity in a dose-dependent manner, while anthracene and phenanthrene did not show any increase in the enzyme activity.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|